Hey Crystal,
The quads do, in fact, work as a muscle group, so you can't really make one of them work in isolation from the others. You can, however, place more stress or focus on one by varying things like foot position during leg extensions (feet turned in/out/straight up to the ceiling), the width of your stance during squats, length of your stride during lunges, whether or not you use a step or bench for variation with squats and lunges, etc.... BTW- I should add that if you try any of these suggestions start with light weight until your body (tendons, ligaments) get accustomed to them and STOP if you have any pain. Get some professional guidance, especially if you have a pre-existing knee problem.
I think it was Maribeth who said, "change the position, change the exercise" (or something very close to that, anyway) and I couldn't agree more. That's why it's important to do a variety of exercises with a variety of equipment to maximize the development of your ALL muscles (think of all the variations on the bicep curl).
Hope this helps.
FitnessRN