Definition is primarily about reducing the fat layer over your muscles. If you have the amount of muscle you want, in addition to eating clean (ie: avoiding processed foods), I would recommend a rotation similar to the following:
Day 1: full-body weight routine (preferably those that are set up in more of a circuit fashion, with little rest between sets, but working different muslce groups each set to allow for recovery of each muscle group. Jari Love has several workouts set up like this. Another good one is Sculpt 5-6 by Tony Horton.)
Day 2: interval cardio (intervals burn more calories than steady state cardio)
Day 3: Full body circuit (as day 1) or cardio/weights circuit (which also has you moving quickly between exercises)
Day 4: steady state cardio
Day 5: Full body circuit or cardio/weights
Day 6: cardio (another interval, if you have worked up to being able to do them, otherwise, steady state)
Day 7: rest
FYI: "Day 1" can start any day of the week, just make it work for you.
If you still want to build more muscle, I recommend replacing two of the full-body weight workouts with a 2-day split (upper body on one day, lower on the other).